Gene sequencing technology has moved beyond laboratory research and become a core engine for precision medicine, life sciences research and agricultural modernization. It supports precise early warning for tumor early screening, rapid infectious disease tracing, molecular breeding of superior crop varieties, and root cause investigation of genetic disorders. With the accelerated adoption of NGS and the gradual application of third- and fourth-generation sequencing technologies, the global gene sequencing market continues to expand.
In the sample preparation stage of sequencing: from nucleic acid extraction, fragmentation, and end repair to adapter ligation, PCR amplification, product purification, size selection, hybrid capture, concentration quality control, and library pooling — every step directly determines the accuracy and reliability of subsequent sequencing data. Traditional manual operations bear challenges from high-throughput batch processing and frequent sample transfers across different instruments, introducing human errors, irregular library quality and cross-contamination that consumes precious samples; such cumbersome workflows severely impair the efficiency and quality of high-throughput sequencing.
To address the key challenges in the sample preparation stage of the gene sequencing workflow, LivoBot has launched its "Automated Platform for Sequencing Sample Preparation (NGS- APW Integrated Fragmentation- to- Library Solution)". With a fully automated, highly integrated product portfolio, this solution helps laboratories overcome the "first critical barrier" of gene sequencing — covering the entire sample preparation workflow from nucleic acid fragmentation to library preparation — delivering both accuracy and high efficiency.
1. Precise Sample Fragmentation
Nucleic acid fragmentation is a critical step in library construction. Uneven fragment sizes and base sequence bias directly affect the quality of sequencing data. LivoBot's EoSonics® FRAG Series Focused Ultrasonicator utilizes non-contact ACU™ (Adaptive Cylindrical Ultrasonic) focusing technology, using physical ultrasonic shearing to effectively overcome the drawbacks of traditional enzymatic fragmentation methods and closed consumable- based ultrasonication methods.
（1）Full high-throughput capability for nucleic acid fragmentation
（2）Single-row processing mode allowing different fragmentation parameters to be set per row
（3）Compatible with general consumables such as 8-tube strips and 96-well plates
（4）No frequent plate transfer required; samples can be directly loaded onto the instrument for fragmentation
（5）Optional dedicated consumables available, eliminating the centrifugation step
2. Automated Library Preparation
LivoBot's EosBot® Seq Series Fully Automated NGS Library Preparation System condenses the entire manual workflow into one instrument. It features a liftable magnetic stand for bead purification and size selection, and integrates a PCR module and concentration quantification module. This keeps samples on-deck for uninterrupted, contamination-free library prep, fully automating both construction and targeted capture.
（1）Library preparation, concentration QC, and normalization — all in one
（2）Externally sealed pipetting module for fast, accurate liquid transfer
（3）Full coverage of medium-to-high throughput, accommodating multiple experimental scales
（4）Diverse module configuration to meet all-scenario needs
3. Application Cases
(1) EoSonics® FRAG Nucleic Acid Fragmentation
The EoSonics® FRAG Series fragments FFPE nucleic acids for TSO500 library prep and sequencing, with results compared to the Illumina-recommended protocol.
Downsampled to equal data, LivoBot matched the reference protocol across all metrics, producing 200–300 bp genomic DNA fragments suitable for direct high-throughput sequencing or bisulfite conversion for methylation analysis.
(2) EosBot® Seq Series Automated Library Preparation
Using a NIPT hybridization capture library preparation kit, 25 μL of cfDNA was input. Libraries were constructed with the EosBot® Seq Series Fully Automated NGS Library Preparation System, and the results were compared with those from manual library preparation. The following results were obtained.
Automated library prep mean conc: 61.3 ng/μL; manual: 63.4 ng/μL (3% difference).
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