Custom Hybridization Capture panels
-based on the TargetSeq® Hybridization Capture Sequencing Technology
https://www.igenetech.com/products/custom-hybridization-capture-panels/
#Hybridization #Capture
Overview
TargetSeq® hybridization capture sequencing technology uses probe hybridization to capture the target regions to detect genetic and tumor-related variation information. TargetSeq® adopts the "stacked tile" probe design strategy to design multi-layer probes in the target area. Meanwhi... moreCustom Hybridization Capture panels
-based on the TargetSeq® Hybridization Capture Sequencing Technology
https://www.igenetech.com/products/custom-hybridization-capture-panels/
#Hybridization #Capture
Overview
TargetSeq® hybridization capture sequencing technology uses probe hybridization to capture the target regions to detect genetic and tumor-related variation information. TargetSeq® adopts the "stacked tile" probe design strategy to design multi-layer probes in the target area. Meanwhile, efficient and uniform data performance can be obtained by using the self-developed and easy-to-use TargetSeq One® hybridization capture reagent kits.
Product Detail
Workflow
TargetSeq® hybrid capture sequencing first requires the construction of a complete library, and then hybridization of the probe with the library to form hybridization products, followed by the use of streptavidin-coated magnetic beads for specific capture of library fragments with targeted sequences. After elution, amplification, purification and other steps, the enriched library of targeted sequences is obtained. When passing the quality control, the final library can be sequenced by using NGS platforms such as Illumina and MGI.
Advantages
Advanced Design Concept
Higher effective coverage results from probe design, which is based on thermodynamic stability and special design scheme for hard-to-capture regions.
Rich Experience in Panel Development
Over 1000 customizd TargetSeq® panels development experience, covering multiple application fields such as tumor companion diagnosis, drug evaluation, genetic screening, molecular breeding, and animal and plant evolution.
Flexible Panel Design
Suitable for target capture of region sizes of 1 kb to 100 Mb. Can detect SNV, InDel, CNV, STR, Fusion and other mutations in the target area. Can also design probes according to the type of mutation.
Outstanding Capture workflow
TargetSeq One® process only requires one single tube operation, which is simple, robust and compatible to different requirements.
Excellent Data Performance
Optimization of key product metrics prior to panel delivery for good performance of coverage rate and uniformity.
High Accuracy
Accurate detection of mutation frequency greater than 1% with good repeatability. With UMI technology, the panels can be used for low-frequency mutation detection as low as 0.1%.
Applications
TargetSeq® hybridization capture sequencing technology is applicable to human, plant and animal, microbial, and other species with known genome. It is suitable for target capture of region sizes of 1 kb to 100 Mb. It can detect SNV, InDel, CNV, Fusion and other variants in the target region, and can be used for the detection of exogenous viruses and viral integration sites analysis. TargetSeq® panels are widely used in application fields such as oncology, genetic disorders, DTC genetic tests, immunology, microbiology, agriculture, and forensic science.
Solutions
iGeneTech Bioscience provides both catalog and customized panels with reagent kits and automation upgrade solutions for the whole TargetSeq® workflow.
3× Tiling Probe Design
The TargetSeq®️ probe is designed with 3× tiling design scheme based on thermodynamic stability to achieve better coverage of target regions. At the same time, there are complete design schemes for reference genome fix or novel patches, hard-to-capture regions, different variant types, CNV, etc.
Customized and Semi-Customized Solutions
With over 1000 TargetSeq®️ customized panel development experience in different application fields, TargetSeq®️ customized panels can meet the diverse needs of our customers. Customers can build their own customized TargetSeq®️ panels or build a semi-customized panels based on pre-designed TargetSeq®️ catalog panels with probes of extra selected regions or probes of some regions to be enhanced.
Simple and Compatible TargetSeq One® Workflow
Simple-to-use TargetSeq One® workflow uses one single-tube operation and requires only two washing reagents during the whole workflow. The reagent kit is compatible with both DNA and RNA probes and can be used for single-hybridization, multiplex-hybridization, and fast-hybridization. It is stable, excellent in performance and cost-effective.
Simple and Compatible TargetSeq One®️ Workflow.png
Excellent Fast-Hybridization Performance
TargetSeq One® workflow is compatible with both fast and overnight hybridization strategy, providing excellent performance to meet the demands of good results with fast speed.
